Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa
نویسندگان
چکیده
Emergomyces africanus is a thermally dimorphic fungus that causes a systemic mycosis in immunocompromised persons in South Africa. Infection is presumed to follow inhalation of airborne propagules. We developed a quantitative PCR protocol able to detect as few as 5 Es. africanus propagules per day. Samples were collected in Cape Town, South Africa over 50 weeks by a Burkard spore trap with an alternate orifice. We detected Es. africanus in air samples from 34 days (10%) distributed over 11 weeks. These results suggest environmental exposure to airborne Es. africanus propagules occurs more commonly in endemic areas than previously appreciated.
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1 Department of Medicine, University of Cape Town, Cape Town, Western Cape, South Africa, 2 Department of Dermatology, University of Cape Town, Cape Town, Western Cape, South Africa, 3 Department of Anatomical Pathology, National Health Laboratory Service Groote Schuur Hospital Branch, Cape Town, Western Cape, South Africa, 4 Division of Infectious Diseases & HIV Medicine, Department of Medicin...
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